Journal: medRxiv

Article Title: Polymorphism in IFNAR contributes to glucocorticoid response and outcome in ARDS and COVID-19

doi: 10.1101/2022.03.10.22272123

Figure Lengend Snippet: (A ) STAT1 expression in the lung after 4-day culture in the presence of IFN beta with or without hydrocortisone (HC). ( B) pSTAT1 expression in the same specimens as in A. ( C) Example photomicrographs showing higher STAT2 expression in a TT patient than in a CT patient and the effect of HC on its nuclear translocation. Most STAT2 remains in the cytoplasm of the CT patients, whereas nuclear expression is prominent in the TT patient. Indicated insets are shown in the bottom row. Arrows. ( D ) Combined results of all patients noting that two CT samples are excluded in the data as the patients were already under glucocorticoid treatment at the time of sample acquisition. Ns, not significant; *P<0.05; **P<0.01; and ***P<0.001

Article Snippet: The first stage antibodies were anti-alpha chain of the IFN alpha/beta receptor (St John’s Laboratory STJ112765) that was used 1:2000 and 1:5000 and anti-Stat1 (1:400, 9175S), anti-pStat1(1:100, 9167S) and anti-Stat2 (1:200, 72604S) all from Cell Signalling.

Techniques: Expressing, Translocation Assay

Journal: Cell

Article Title: Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease

doi: 10.1016/j.cell.2018.08.067

Figure Lengend Snippet:

Article Snippet: Slides were washed in PBS containing 0.05% Tween 20 (PBS-T) and incubated with ImmPRESS HRP Anti-Rabbit or Anti-Mouse IgG (Peroxidase) Polymer Detection Kit (Vector Laboratories, Peterborough, U.K) for thirty minutes at room temperature.

Techniques: Recombinant, Coagulation, Antibody Labeling, Plasmid Preparation, Blocking Assay, Staining, Imaging, Software, Hybridization

Journal: Genome Biology

Article Title: The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins

doi: 10.1186/gb-2006-7-9-r80

Figure Lengend Snippet: Urinary protein separation by one-dimensional SDS gel and reverse-phase HPLC. (a) 150 μg urinary protein (25 μg/lane) from single sample and pooled sample were applied on a 4-12% Bis-Tris gel. Gel was stained by colloidal Coomassie and cut into 14 pieces (in-gel 1 set) or 10 pieces (in-gel 2 set) for single urine sample, and cut into 10 pieces for pooled urine sample. (b) 250 μg of urinary protein was applied to Vivapure Anti-HSA Kit to deplete serum albumin. The albumin-depleted protein mixture was dissolved in 6 mol/l urea and 1.0% acetic acid solution, and separated on mRP-C18 High-Recovery protein column at 80°C using linear multi-segment gradient, as described in the Materials and Methods section. HPLC, high-performance liquid chromatography; SDS, sodium dodecyl sulfate.

Article Snippet: Protein (250 μg) was applied to Vivapure Anti-HSA Kit (Vivascience, Hanover, Germany) to deplete serum albumin.

Techniques: SDS-Gel, Staining, High Performance Liquid Chromatography